| 品牌： | NEWLF  |
| 價(jià)格： | ￥4480.00 |
| 規(guī)格： | 100ul |
| 貨號： | NL25117 |
| 應(yīng)用： | ELISA WB IF |
Product Description
| Description | Anti-RHEB (Y35N) mouse monoclonal antibody |
| Another Name | Anti-RHEB (mutated Y35N) mouse monoclonal antibody |
| Catalog Number | NL25117 |
| Size: | 100 μl |
| Tested Applications: | ELISA WB IF IHC ELISA:1:500-1:1000 WB:1:500-1:1000 IF:1:50-1:100 |
| Cited Applications: | WB IF |
| Tested Reactivity: | Human , mouse Rat |
| Cited Reactivity: | Human , mouse Rat |
| Gene Symbol: | RHEB2 |
| Immunogen : | A synthetic peptide from the internal region of human RHEB which includes the mutation of Y35N |
| Concentration: | 1mg/ml |
| Host/Isotype: | mouse/IgG |
| Class: | monnoclonal |
| Type: | Antibody |
| Predicted mv: | 20 KDa |
| Observed mv: | 20 KDa |
| Uniprot: | Q15382 |
| Constituents: | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Storage Conditions: | Store at -20°C for one year after shipment |
Background: | This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. |
Tested Applications: WB
Tested Applications: IF
Immunofluorescence of cells expressing RHEB proteins with Anti RHEB(Y35N) antibody. HEK293T cells were transfected with pCDNA3-GFP RHEB(WT) plasmid (left column) or pCDNA3-GFP RHEB(Y35N) plasmid (right column), then fixed and stained with Anti RHEB(Y35N) monoclonal antibody (Cat. #NL25117).
實(shí)驗(yàn)方案
| IHC protocol for Rac1-GTP antibody NL23001 Download protocol | |
| IF protocol for Rac1-GTP antibody NL23001 Download protocol | |
| WB protocol for Rac1 antibody NL24001 Download protocol |
操作指南
| 免疫印跡實(shí)驗(yàn)操作指南 Download protocol | |
| 免疫組化實(shí)驗(yàn)操作指南 Download protocol | |
| 免疫沉淀實(shí)驗(yàn)操作指南 Download protocol | |
| 免疫細(xì)胞化學(xué)實(shí)驗(yàn)操作指南 Download protocol | |
| 免疫熒光實(shí)驗(yàn)操作指南 Download protocol |
操作指南
| 免疫印跡實(shí)驗(yàn)操作指南 Download protocol | |
| 免疫組化實(shí)驗(yàn)操作指南 Download protocol | |
| 免疫沉淀實(shí)驗(yàn)操作指南 Download protocol | |
| 免疫細(xì)胞化學(xué)實(shí)驗(yàn)操作指南 Download protocol | |
| 免疫熒光實(shí)驗(yàn)操作指南 Download protocol |
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